Queen B, who made history by becoming the first solo female Pyramid stage headliner in twenty years, opened her set with Crazy In Love, as she told the crowd: "I want y'all to know that right now, you're witnessing my dream,"
She then powered her way through hits such as Single Ladies, encouraging the 175,000 strong crowd to sing-a-long with her, before welcoming special guestTricky on stage to help her sing Baby Boy.
After wishing her friend Steve Style a happy birthday, Bey performed her new single, Best Thing I Never Had, 4 album track, End Of Time and If I Were A Boy, mashed-up with the Alanis Morissette classic, You Ougtha Know.
SEE MORE BOOTYLICIOUS SNAPS OF BEYONCE AT GLASTO HERE!
The 29-year old diva, who releases her fourth studio album, 4, today, then covered Prince's The Beautiful Ones and Kings Of Leon's Sex On Fire, laying seductively on the stage as she belted out the raunchy lyrics.
Beyoncé then asked the crowd to help her sing Irreplaceable, telling her audience: "I've done a lot of things in my life but I've never performed in front of 175,000 people. There hasn't been a woman headlining Glastonbury for 20 years, so this is history for me,"
Wearing a glitzy gold blazer - which just about covered her bootylicious behind, Knowles then launched into a Destiny's Child medley, including hits Say My Name, Survivor and Jumpin' Jumpin', as well as singing her part on Lady GaGa's No.1 hit, Telephone.
Finishing with Run The World (Girls) and Halo - where footballer Wayne Rooneybizarrely featured on the screen behind her - the star disappeared into the Pyramid stage.
Speaking after her set, Beyoncé told the BBC: "This night was a dream. I felt like a rockstar, and the crowd, they looked like rockstars, and I'm just so honoured, really. This is... the highlight of my career."
Bey continued: "I'm so happy because, you know, I don't normally do festivals and 175,000 people and... I was very nervous. Everyone gave me so much love and I will never, ever, ever forget this night."
Mrs. Jay-Z also revealed that Coldplay frontman Chris Martin "approved" her set list, stating that "he's the master of this."
Beyoncé's 2011 Glastonbury setlist:
1. Crazy In Love 2. Single Ladies (Put A Ring On It) 3. Naughty Girl 4. Baby Boy 5. Best Thing I Never Had 6. End Of Time 7. If I Were A Boy/You Oughta Know 8. Sweet Dreams 9. Why Don't You Love Me? 10. The Beautiful Ones 11. Sex On Fire 12. 1 + 1 13. Irreplacable 14. Destiny's Child and Lady GaGa medley: Independent Women Part 1/ Bootylicious / Bug A Boo / Telephone / Say My Name / Jumpin', Jumpin'/ Survivor 15. At Last 16. Run The World (Girls) 17. Halo