Ronson, who attended the Rehab singer's funeral last week before hitting the White Isle, took a moment to remember the 27-year old during his set at super-club, Amnesia - playing a moving rendition of Back To Black - the song they wrote together.
Paying homage to Amy during his set, Mark told the crowd: "I don't wanna get super deep and morbid here, but she was a great friend of mine, a great f**king musician and somebody that gave us a s**t load a brilliant music through all of our lives…"
"She left us this week but her music is gonna be with us forever, that's some f**king good s**t and the good s**t is, that I can lose somebody whose f**king like a sister to me and then come here and play music for you and feel better about everything, as music is the f**king healer so this is a pleasure."
He continued: "What I'd like to say to you is, if you are a fan of the music of Amy Winehouse and if she meant anything to you, you should make some noise right now. The first song that me and Amy ever wrote together was a song called Back To Black."
Ronson went on to explain the reason whyThe Rumble Strips frontman, Charlie Waller, would be singing Winehouse's iconic tune for the Amnesia partygoers.
He revealed: "Amy famously never liked anything. Before I met him, Charlie and his band did a cover of this song that me and Amy wrote and I remember playing it to Amy one time and saying 'check out this cover of a band I kinda like did' and she goes 'yeah, it's the nuts' - that was one of her favourite expressions.
Check out Mark Ronson And The Business Intl's headline stint on MTV Music, Saturday 6th August at 9pm.